Transformation

The journey of transformation of bacteria. This transformation is an amazing journey in biology. I had a journey while experimenting with a plasmid in the laboratory of class in the natural science campus called general Biology. I had to make an experiment which involved transforming the plasmid in order to find out if it gave the bacteria a resistance to antibiotics and manage to activate B-galactosidasa. In this journey I had to prove that we can use plasmid like vectors to facilitate the transmission and expression of a gen in a bacteria. This is made via heat shock procedure which consists in inducting a bacteria to a dramatic change in temperature in order to weaken the membrane. This was proven thanks to the D N A this making the whole process possible. Of course we also needed the puc which contains the gene lac Z which codifies the enzyme B galactosidasa and in turn catalyzes the degradation of lactose and its analogues in presence of an inductor. At the end the bacteria turned blue. The control group also exhibit growth in the form of colonies of bacteria’s but that means that there was an error in the handling of the control group because it was not supposed to exhibit anything at all. The experimental group was made correct because it turned the color that it was supposed to turn. The manipulation of a gene in a bacteria using a plasmid and heat shock was an amazing experience for me and an amazing journey in the process of DNA extraction which has revolutionize science. I look forward to having more experience like this in the field of Biology and in the field of medical science.